HEM15/YOR176W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 25 6.4%
Arg[R] 16 4.1%
Asn[N] 16 4.1%
Asp[D] 18 4.6%
Cys[C] 3 0.8%
Gln[Q] 21 5.3%
Glu[E] 23 5.9%
Gly[G] 21 5.3%
His[H] 7 1.8%
Ile[I] 25 6.4%
Leu[L] 33 8.4%
Lys[K] 33 8.4%
Met[M] 9 2.3%
Phe[F] 20 5.1%
Pro[P] 22 5.6%
Ser[S] 32 8.1%
Thr[T] 25 6.4%
Trp[W] 6 1.5%
Tyr[Y] 16 4.1%
Val[V] 22 5.6%
Total Length 393

Instability index
Instability index (II) 41.80
This classifies the protein as unstable

Atomic composition
Carbon 2017
Hydrogen 3147
Nitrogen 531
Oxygen 586
Sulfur 12
Total number of atoms 6293
Formula = C2017H3147N531O586S12

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 56965 M-1 cm-1
assuming NO Cys residues appear as half cystines 56840 M-1 cm-1
assuming all Cys residues are reduced 56840 M-1 cm-1
assuming all pairs of Cys residues form cystines 56965 M-1 cm-1

Aliphatic index
Aliphatic index 80.15

Coding Region Translation Calculations
Codon Bias 0.119
Codon Adaptation Index 0.169
Frequency of Optimal Codons 0.479
Hydropathicity of Protein -0.390
Aromaticity Score 0.107