Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
39 |
5.0% |
| Arg[R] |
35 |
4.5% |
| Asn[N] |
37 |
4.8% |
| Asp[D] |
57 |
7.3% |
| Cys[C] |
5 |
0.6% |
| Gln[Q] |
30 |
3.9% |
| Glu[E] |
74 |
9.5% |
| Gly[G] |
34 |
4.4% |
| His[H] |
19 |
2.4% |
| Ile[I] |
53 |
6.8% |
| Leu[L] |
78 |
10.1% |
| Lys[K] |
63 |
8.1% |
| Met[M] |
12 |
1.5% |
| Phe[F] |
40 |
5.2% |
| Pro[P] |
17 |
2.2% |
| Ser[S] |
52 |
6.7% |
| Thr[T] |
42 |
5.4% |
| Trp[W] |
12 |
1.5% |
| Tyr[Y] |
20 |
2.6% |
| Val[V] |
57 |
7.3% |
| Total Length |
776 |
|
| Instability index (II) |
42.30 |
| This classifies the protein as unstable |
|
| Carbon |
4010 |
| Hydrogen |
6293 |
| Nitrogen |
1061 |
| Oxygen |
1220 |
| Sulfur |
17 |
| Total number of atoms |
12601 |
| Formula = C4010H6293N1061O1220S17 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
96050 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
95800 M-1 cm-1 |
| assuming all Cys residues are reduced |
95800 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
96050 M-1 cm-1 |
|
|
| Codon Bias |
0.092 |
| Codon Adaptation Index |
0.182 |
| Frequency of Optimal Codons |
0.473 |
| Hydropathicity of Protein |
-0.404 |
| Aromaticity Score |
0.093 |
|
