Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
120 |
7.9% |
| Arg[R] |
68 |
4.5% |
| Asn[N] |
70 |
4.6% |
| Asp[D] |
66 |
4.4% |
| Cys[C] |
28 |
1.9% |
| Gln[Q] |
56 |
3.7% |
| Glu[E] |
84 |
5.6% |
| Gly[G] |
94 |
6.2% |
| His[H] |
25 |
1.7% |
| Ile[I] |
88 |
5.8% |
| Leu[L] |
134 |
8.9% |
| Lys[K] |
87 |
5.8% |
| Met[M] |
42 |
2.8% |
| Phe[F] |
90 |
6.0% |
| Pro[P] |
59 |
3.9% |
| Ser[S] |
126 |
8.3% |
| Thr[T] |
81 |
5.4% |
| Trp[W] |
23 |
1.5% |
| Tyr[Y] |
77 |
5.1% |
| Val[V] |
93 |
6.2% |
| Total Length |
1511 |
|
| Instability index (II) |
35.95 |
| This classifies the protein as stable |
|
| Carbon |
7716 |
| Hydrogen |
11845 |
| Nitrogen |
2001 |
| Oxygen |
2222 |
| Sulfur |
70 |
| Total number of atoms |
23854 |
| Formula = C7716H11845N2001O2222S70 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
242980 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
241230 M-1 cm-1 |
| assuming all Cys residues are reduced |
241230 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
242980 M-1 cm-1 |
|
|
| Codon Bias |
0.277 |
| Codon Adaptation Index |
0.246 |
| Frequency of Optimal Codons |
0.568 |
| Hydropathicity of Protein |
-0.124 |
| Aromaticity Score |
0.126 |
|