Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
80 |
6.5% |
| Arg[R] |
79 |
6.5% |
| Asn[N] |
41 |
3.3% |
| Asp[D] |
77 |
6.3% |
| Cys[C] |
17 |
1.4% |
| Gln[Q] |
48 |
3.9% |
| Glu[E] |
93 |
7.6% |
| Gly[G] |
83 |
6.8% |
| His[H] |
33 |
2.7% |
| Ile[I] |
91 |
7.4% |
| Leu[L] |
106 |
8.7% |
| Lys[K] |
74 |
6.0% |
| Met[M] |
40 |
3.3% |
| Phe[F] |
50 |
4.1% |
| Pro[P] |
53 |
4.3% |
| Ser[S] |
75 |
6.1% |
| Thr[T] |
70 |
5.7% |
| Trp[W] |
7 |
0.6% |
| Tyr[Y] |
46 |
3.8% |
| Val[V] |
61 |
5.0% |
| Total Length |
1224 |
|
| Instability index (II) |
44.02 |
| This classifies the protein as unstable |
|
| Carbon |
6148 |
| Hydrogen |
9715 |
| Nitrogen |
1697 |
| Oxygen |
1845 |
| Sulfur |
57 |
| Total number of atoms |
19462 |
| Formula = C6148H9715N1697O1845S57 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
108040 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
107040 M-1 cm-1 |
| assuming all Cys residues are reduced |
107040 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
108040 M-1 cm-1 |
|
|
| Codon Bias |
0.205 |
| Codon Adaptation Index |
0.228 |
| Frequency of Optimal Codons |
0.526 |
| Hydropathicity of Protein |
-0.391 |
| Aromaticity Score |
0.084 |
|
