Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
29 |
3.7% |
| Arg[R] |
42 |
5.3% |
| Asn[N] |
57 |
7.2% |
| Asp[D] |
62 |
7.8% |
| Cys[C] |
8 |
1.0% |
| Gln[Q] |
30 |
3.8% |
| Glu[E] |
52 |
6.6% |
| Gly[G] |
17 |
2.1% |
| His[H] |
21 |
2.7% |
| Ile[I] |
55 |
7.0% |
| Leu[L] |
89 |
11.3% |
| Lys[K] |
67 |
8.5% |
| Met[M] |
11 |
1.4% |
| Phe[F] |
31 |
3.9% |
| Pro[P] |
31 |
3.9% |
| Ser[S] |
72 |
9.1% |
| Thr[T] |
47 |
5.9% |
| Trp[W] |
7 |
0.9% |
| Tyr[Y] |
20 |
2.5% |
| Val[V] |
43 |
5.4% |
| Total Length |
791 |
|
| Instability index (II) |
41.52 |
| This classifies the protein as unstable |
|
| Carbon |
4040 |
| Hydrogen |
6454 |
| Nitrogen |
1120 |
| Oxygen |
1246 |
| Sulfur |
19 |
| Total number of atoms |
12879 |
| Formula = C4040H6454N1120O1246S19 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
68800 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
68300 M-1 cm-1 |
| assuming all Cys residues are reduced |
68300 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
68800 M-1 cm-1 |
|
|
| Codon Bias |
0.005 |
| Codon Adaptation Index |
0.134 |
| Frequency of Optimal Codons |
0.418 |
| Hydropathicity of Protein |
-0.574 |
| Aromaticity Score |
0.073 |
|
