NUP1/YOR098C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 77 7.2%
Arg[R] 30 2.8%
Asn[N] 100 9.3%
Asp[D] 39 3.6%
Cys[C] 2 0.2%
Gln[Q] 31 2.9%
Glu[E] 52 4.8%
Gly[G] 87 8.1%
His[H] 9 0.8%
Ile[I] 32 3.0%
Leu[L] 42 3.9%
Lys[K] 93 8.6%
Met[M] 12 1.1%
Phe[F] 71 6.6%
Pro[P] 77 7.2%
Ser[S] 161 15.0%
Thr[T] 118 11.0%
Trp[W] 1 0.1%
Tyr[Y] 5 0.5%
Val[V] 37 3.4%
Total Length 1076

Instability index
Instability index (II) 42.18
This classifies the protein as unstable

Atomic composition
Carbon 4898
Hydrogen 7723
Nitrogen 1409
Oxygen 1674
Sulfur 14
Total number of atoms 15718
Formula = C4898H7723N1409O1674S14

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 13075 M-1 cm-1
assuming NO Cys residues appear as half cystines 12950 M-1 cm-1
assuming all Cys residues are reduced 12950 M-1 cm-1
assuming all pairs of Cys residues form cystines 13075 M-1 cm-1

Aliphatic index
Aliphatic index 43.95

Coding Region Translation Calculations
Codon Bias 0.024
Codon Adaptation Index 0.121
Frequency of Optimal Codons 0.415
Hydropathicity of Protein -0.796
Aromaticity Score 0.072