SKI7/YOR076C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 24 3.2%
Arg[R] 17 2.3%
Asn[N] 66 8.8%
Asp[D] 40 5.4%
Cys[C] 8 1.1%
Gln[Q] 26 3.5%
Glu[E] 49 6.6%
Gly[G] 21 2.8%
His[H] 20 2.7%
Ile[I] 63 8.4%
Leu[L] 95 12.7%
Lys[K] 73 9.8%
Met[M] 8 1.1%
Phe[F] 27 3.6%
Pro[P] 31 4.1%
Ser[S] 76 10.2%
Thr[T] 46 6.2%
Trp[W] 5 0.7%
Tyr[Y] 19 2.5%
Val[V] 33 4.4%
Total Length 747

Instability index
Instability index (II) 39.85
This classifies the protein as stable

Atomic composition
Carbon 3786
Hydrogen 6082
Nitrogen 1008
Oxygen 1159
Sulfur 16
Total number of atoms 12051
Formula = C3786H6082N1008O1159S16

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 56310 M-1 cm-1
assuming NO Cys residues appear as half cystines 55810 M-1 cm-1
assuming all Cys residues are reduced 55810 M-1 cm-1
assuming all pairs of Cys residues form cystines 56310 M-1 cm-1

Aliphatic index
Aliphatic index 98.51

Coding Region Translation Calculations
Codon Bias -0.004
Codon Adaptation Index 0.132
Frequency of Optimal Codons 0.418
Hydropathicity of Protein -0.404
Aromaticity Score 0.068