Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
39 |
5.8% |
| Arg[R] |
32 |
4.7% |
| Asn[N] |
44 |
6.5% |
| Asp[D] |
46 |
6.8% |
| Cys[C] |
2 |
0.3% |
| Gln[Q] |
27 |
4.0% |
| Glu[E] |
70 |
10.4% |
| Gly[G] |
29 |
4.3% |
| His[H] |
9 |
1.3% |
| Ile[I] |
36 |
5.3% |
| Leu[L] |
60 |
8.9% |
| Lys[K] |
59 |
8.7% |
| Met[M] |
11 |
1.6% |
| Phe[F] |
32 |
4.7% |
| Pro[P] |
25 |
3.7% |
| Ser[S] |
70 |
10.4% |
| Thr[T] |
35 |
5.2% |
| Trp[W] |
6 |
0.9% |
| Tyr[Y] |
12 |
1.8% |
| Val[V] |
31 |
4.6% |
| Total Length |
675 |
|
| Instability index (II) |
45.03 |
| This classifies the protein as unstable |
|
| Carbon |
3349 |
| Hydrogen |
5309 |
| Nitrogen |
925 |
| Oxygen |
1096 |
| Sulfur |
13 |
| Total number of atoms |
10692 |
| Formula = C3349H5309N925O1096S13 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
51005 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
50880 M-1 cm-1 |
| assuming all Cys residues are reduced |
50880 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
51005 M-1 cm-1 |
|
|
| Codon Bias |
0.061 |
| Codon Adaptation Index |
0.137 |
| Frequency of Optimal Codons |
0.445 |
| Hydropathicity of Protein |
-0.748 |
| Aromaticity Score |
0.074 |
|
