TOM6/YOR045W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 12 19.7%
Arg[R] 1 1.6%
Asn[N] 1 1.6%
Asp[D] 2 3.3%
Cys[C] 0 0.0%
Gln[Q] 5 8.2%
Glu[E] 1 1.6%
Gly[G] 5 8.2%
His[H] 0 0.0%
Ile[I] 2 3.3%
Leu[L] 5 8.2%
Lys[K] 2 3.3%
Met[M] 5 8.2%
Phe[F] 6 9.8%
Pro[P] 6 9.8%
Ser[S] 4 6.6%
Thr[T] 1 1.6%
Trp[W] 0 0.0%
Tyr[Y] 1 1.6%
Val[V] 2 3.3%
Total Length 61

Instability index
Instability index (II) 57.46
This classifies the protein as unstable

Atomic composition
Carbon 292
Hydrogen 448
Nitrogen 72
Oxygen 80
Sulfur 5
Total number of atoms 897
Formula = C292H448N72O80S5

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 1490 M-1 cm-1

Aliphatic index
Aliphatic index 73.93

Coding Region Translation Calculations
Codon Bias 0.473
Codon Adaptation Index 0.301
Frequency of Optimal Codons 0.661
Hydropathicity of Protein 0.389
Aromaticity Score 0.115