CRS5/YOR031W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 0 0.0%
Arg[R] 0 0.0%
Asn[N] 1 1.5%
Asp[D] 3 4.4%
Cys[C] 19 27.9%
Gln[Q] 1 1.5%
Glu[E] 6 8.8%
Gly[G] 6 8.8%
His[H] 2 2.9%
Ile[I] 1 1.5%
Leu[L] 1 1.5%
Lys[K] 10 14.7%
Met[M] 1 1.5%
Phe[F] 0 0.0%
Pro[P] 2 2.9%
Ser[S] 8 11.8%
Thr[T] 6 8.8%
Trp[W] 0 0.0%
Tyr[Y] 0 0.0%
Val[V] 1 1.5%
Total Length 68

Instability index
Instability index (II) 60.19
This classifies the protein as unstable

Atomic composition
Carbon 272
Hydrogen 456
Nitrogen 84
Oxygen 103
Sulfur 20
Total number of atoms 935
Formula = C272H456N84O103S20

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 1125 M-1 cm-1
assuming NO Cys residues appear as half cystines 0 M-1 cm-1
assuming all Cys residues are reduced 0 M-1 cm-1
assuming all pairs of Cys residues form cystines 1125 M-1 cm-1

Aliphatic index
Aliphatic index 15.74

Coding Region Translation Calculations
Codon Bias 0.189
Codon Adaptation Index 0.228
Frequency of Optimal Codons 0.552
Hydropathicity of Protein -0.562
Aromaticity Score 0.000