Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
89 |
6.4% |
| Arg[R] |
43 |
3.1% |
| Asn[N] |
70 |
5.0% |
| Asp[D] |
70 |
5.0% |
| Cys[C] |
26 |
1.9% |
| Gln[Q] |
53 |
3.8% |
| Glu[E] |
59 |
4.2% |
| Gly[G] |
77 |
5.5% |
| His[H] |
26 |
1.9% |
| Ile[I] |
94 |
6.7% |
| Leu[L] |
145 |
10.4% |
| Lys[K] |
83 |
6.0% |
| Met[M] |
27 |
1.9% |
| Phe[F] |
103 |
7.4% |
| Pro[P] |
48 |
3.4% |
| Ser[S] |
125 |
9.0% |
| Thr[T] |
86 |
6.2% |
| Trp[W] |
21 |
1.5% |
| Tyr[Y] |
67 |
4.8% |
| Val[V] |
82 |
5.9% |
| Total Length |
1394 |
|
| Instability index (II) |
42.33 |
| This classifies the protein as unstable |
|
| Carbon |
7230 |
| Hydrogen |
11022 |
| Nitrogen |
1802 |
| Oxygen |
2054 |
| Sulfur |
53 |
| Total number of atoms |
22161 |
| Formula = C7230H11022N1802O2054S53 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
216955 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
215330 M-1 cm-1 |
| assuming all Cys residues are reduced |
215330 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
216955 M-1 cm-1 |
|
|
| Codon Bias |
0.191 |
| Codon Adaptation Index |
0.196 |
| Frequency of Optimal Codons |
0.522 |
| Hydropathicity of Protein |
0.019 |
| Aromaticity Score |
0.137 |
|
