Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
63 |
12.9% |
| Arg[R] |
2 |
0.4% |
| Asn[N] |
12 |
2.5% |
| Asp[D] |
13 |
2.7% |
| Cys[C] |
5 |
1.0% |
| Gln[Q] |
9 |
1.8% |
| Glu[E] |
24 |
4.9% |
| Gly[G] |
11 |
2.3% |
| His[H] |
1 |
0.2% |
| Ile[I] |
16 |
3.3% |
| Leu[L] |
19 |
3.9% |
| Lys[K] |
10 |
2.1% |
| Met[M] |
8 |
1.6% |
| Phe[F] |
3 |
0.6% |
| Pro[P] |
18 |
3.7% |
| Ser[S] |
149 |
30.6% |
| Thr[T] |
52 |
10.7% |
| Trp[W] |
1 |
0.2% |
| Tyr[Y] |
11 |
2.3% |
| Val[V] |
60 |
12.3% |
| Total Length |
487 |
|
| Instability index (II) |
61.23 |
| This classifies the protein as unstable |
|
| Carbon |
2001 |
| Hydrogen |
3271 |
| Nitrogen |
527 |
| Oxygen |
795 |
| Sulfur |
13 |
| Total number of atoms |
6607 |
| Formula = C2001H3271N527O795S13 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
22140 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
21890 M-1 cm-1 |
| assuming all Cys residues are reduced |
21890 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
22140 M-1 cm-1 |
|
|
| Codon Bias |
0.516 |
| Codon Adaptation Index |
0.358 |
| Frequency of Optimal Codons |
0.705 |
| Hydropathicity of Protein |
0.180 |
| Aromaticity Score |
0.031 |
|
