Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
35 |
6.4% |
| Arg[R] |
17 |
3.1% |
| Asn[N] |
20 |
3.7% |
| Asp[D] |
15 |
2.8% |
| Cys[C] |
9 |
1.7% |
| Gln[Q] |
16 |
2.9% |
| Glu[E] |
11 |
2.0% |
| Gly[G] |
28 |
5.1% |
| His[H] |
12 |
2.2% |
| Ile[I] |
45 |
8.3% |
| Leu[L] |
73 |
13.4% |
| Lys[K] |
24 |
4.4% |
| Met[M] |
13 |
2.4% |
| Phe[F] |
49 |
9.0% |
| Pro[P] |
34 |
6.2% |
| Ser[S] |
44 |
8.1% |
| Thr[T] |
22 |
4.0% |
| Trp[W] |
16 |
2.9% |
| Tyr[Y] |
35 |
6.4% |
| Val[V] |
26 |
4.8% |
| Total Length |
544 |
|
| Instability index (II) |
45.73 |
| This classifies the protein as unstable |
|
| Carbon |
3006 |
| Hydrogen |
4459 |
| Nitrogen |
695 |
| Oxygen |
734 |
| Sulfur |
22 |
| Total number of atoms |
8916 |
| Formula = C3006H4459N695O734S22 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
140650 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
140150 M-1 cm-1 |
| assuming all Cys residues are reduced |
140150 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
140650 M-1 cm-1 |
|
|
| Codon Bias |
0.024 |
| Codon Adaptation Index |
0.112 |
| Frequency of Optimal Codons |
0.410 |
| Hydropathicity of Protein |
0.432 |
| Aromaticity Score |
0.184 |
|
