Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
42 |
5.7% |
| Arg[R] |
43 |
5.9% |
| Asn[N] |
48 |
6.5% |
| Asp[D] |
52 |
7.1% |
| Cys[C] |
3 |
0.4% |
| Gln[Q] |
20 |
2.7% |
| Glu[E] |
56 |
7.6% |
| Gly[G] |
20 |
2.7% |
| His[H] |
17 |
2.3% |
| Ile[I] |
50 |
6.8% |
| Leu[L] |
78 |
10.6% |
| Lys[K] |
62 |
8.5% |
| Met[M] |
11 |
1.5% |
| Phe[F] |
22 |
3.0% |
| Pro[P] |
44 |
6.0% |
| Ser[S] |
65 |
8.9% |
| Thr[T] |
25 |
3.4% |
| Trp[W] |
7 |
1.0% |
| Tyr[Y] |
26 |
3.5% |
| Val[V] |
42 |
5.7% |
| Total Length |
733 |
|
| Instability index (II) |
45.33 |
| This classifies the protein as unstable |
|
| Carbon |
3744 |
| Hydrogen |
5961 |
| Nitrogen |
1033 |
| Oxygen |
1134 |
| Sulfur |
14 |
| Total number of atoms |
11886 |
| Formula = C3744H5961N1033O1134S14 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
77365 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
77240 M-1 cm-1 |
| assuming all Cys residues are reduced |
77240 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
77365 M-1 cm-1 |
|
|
| Codon Bias |
0.004 |
| Codon Adaptation Index |
0.127 |
| Frequency of Optimal Codons |
0.406 |
| Hydropathicity of Protein |
-0.587 |
| Aromaticity Score |
0.075 |
|
