BDS1/YOL164W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 55 8.5%
Arg[R] 36 5.6%
Asn[N] 34 5.3%
Asp[D] 32 5.0%
Cys[C] 6 0.9%
Gln[Q] 32 5.0%
Glu[E] 44 6.8%
Gly[G] 44 6.8%
His[H] 16 2.5%
Ile[I] 39 6.0%
Leu[L] 54 8.4%
Lys[K] 31 4.8%
Met[M] 18 2.8%
Phe[F] 26 4.0%
Pro[P] 25 3.9%
Ser[S] 36 5.6%
Thr[T] 38 5.9%
Trp[W] 10 1.5%
Tyr[Y] 24 3.7%
Val[V] 46 7.1%
Total Length 646

Instability index
Instability index (II) 42.57
This classifies the protein as unstable

Atomic composition
Carbon 3236
Hydrogen 5053
Nitrogen 893
Oxygen 963
Sulfur 24
Total number of atoms 10169
Formula = C3236H5053N893O963S24

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 91135 M-1 cm-1
assuming NO Cys residues appear as half cystines 90760 M-1 cm-1
assuming all Cys residues are reduced 90760 M-1 cm-1
assuming all pairs of Cys residues form cystines 91135 M-1 cm-1

Aliphatic index
Aliphatic index 85.31

Coding Region Translation Calculations
Codon Bias 0.050
Codon Adaptation Index 0.118
Frequency of Optimal Codons 0.432
Hydropathicity of Protein -0.293
Aromaticity Score 0.093