Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
84 |
8.7% |
| Arg[R] |
3 |
0.3% |
| Asn[N] |
37 |
3.8% |
| Asp[D] |
23 |
2.4% |
| Cys[C] |
13 |
1.3% |
| Gln[Q] |
15 |
1.6% |
| Glu[E] |
46 |
4.8% |
| Gly[G] |
88 |
9.1% |
| His[H] |
5 |
0.5% |
| Ile[I] |
42 |
4.3% |
| Leu[L] |
33 |
3.4% |
| Lys[K] |
21 |
2.2% |
| Met[M] |
1 |
0.1% |
| Phe[F] |
25 |
2.6% |
| Pro[P] |
27 |
2.8% |
| Ser[S] |
255 |
26.4% |
| Thr[T] |
155 |
16.0% |
| Trp[W] |
0 |
0.0% |
| Tyr[Y] |
26 |
2.7% |
| Val[V] |
68 |
7.0% |
| Total Length |
967 |
|
| Instability index (II) |
41.39 |
| This classifies the protein as unstable |
|
| Carbon |
3960 |
| Hydrogen |
6307 |
| Nitrogen |
1059 |
| Oxygen |
1594 |
| Sulfur |
14 |
| Total number of atoms |
12934 |
| Formula = C3960H6307N1059O1594S14 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
39490 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
38740 M-1 cm-1 |
| assuming all Cys residues are reduced |
38740 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
39490 M-1 cm-1 |
|
|
| Codon Bias |
0.411 |
| Codon Adaptation Index |
0.276 |
| Frequency of Optimal Codons |
0.646 |
| Hydropathicity of Protein |
-0.108 |
| Aromaticity Score |
0.053 |
|
