Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
29 |
4.7% |
| Arg[R] |
30 |
4.8% |
| Asn[N] |
23 |
3.7% |
| Asp[D] |
25 |
4.0% |
| Cys[C] |
13 |
2.1% |
| Gln[Q] |
18 |
2.9% |
| Glu[E] |
27 |
4.4% |
| Gly[G] |
38 |
6.1% |
| His[H] |
16 |
2.6% |
| Ile[I] |
56 |
9.0% |
| Leu[L] |
59 |
9.5% |
| Lys[K] |
33 |
5.3% |
| Met[M] |
13 |
2.1% |
| Phe[F] |
40 |
6.5% |
| Pro[P] |
29 |
4.7% |
| Ser[S] |
54 |
8.7% |
| Thr[T] |
28 |
4.5% |
| Trp[W] |
11 |
1.8% |
| Tyr[Y] |
34 |
5.5% |
| Val[V] |
44 |
7.1% |
| Total Length |
620 |
|
| Instability index (II) |
42.47 |
| This classifies the protein as unstable |
|
| Carbon |
3274 |
| Hydrogen |
5013 |
| Nitrogen |
827 |
| Oxygen |
882 |
| Sulfur |
26 |
| Total number of atoms |
10022 |
| Formula = C3274H5013N827O882S26 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
111910 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
111160 M-1 cm-1 |
| assuming all Cys residues are reduced |
111160 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
111910 M-1 cm-1 |
|
|
| Codon Bias |
0.095 |
| Codon Adaptation Index |
0.144 |
| Frequency of Optimal Codons |
0.466 |
| Hydropathicity of Protein |
0.102 |
| Aromaticity Score |
0.137 |
|
