MED7/YOL135C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 6 2.7%
Arg[R] 9 4.1%
Asn[N] 14 6.3%
Asp[D] 7 3.2%
Cys[C] 2 0.9%
Gln[Q] 16 7.2%
Glu[E] 21 9.5%
Gly[G] 8 3.6%
His[H] 4 1.8%
Ile[I] 13 5.9%
Leu[L] 27 12.2%
Lys[K] 18 8.1%
Met[M] 5 2.3%
Phe[F] 3 1.4%
Pro[P] 17 7.7%
Ser[S] 21 9.5%
Thr[T] 10 4.5%
Trp[W] 1 0.5%
Tyr[Y] 12 5.4%
Val[V] 8 3.6%
Total Length 222

Instability index
Instability index (II) 55.69
This classifies the protein as unstable

Atomic composition
Carbon 1134
Hydrogen 1808
Nitrogen 306
Oxygen 352
Sulfur 7
Total number of atoms 3607
Formula = C1134H1808N306O352S7

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 23505 M-1 cm-1
assuming NO Cys residues appear as half cystines 23380 M-1 cm-1
assuming all Cys residues are reduced 23380 M-1 cm-1
assuming all pairs of Cys residues form cystines 23505 M-1 cm-1

Aliphatic index
Aliphatic index 83.42

Coding Region Translation Calculations
Codon Bias 0.001
Codon Adaptation Index 0.089
Frequency of Optimal Codons 0.403
Hydropathicity of Protein -0.760
Aromaticity Score 0.072