RPL25/YOL127W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 19 13.4%
Arg[R] 7 4.9%
Asn[N] 7 4.9%
Asp[D] 6 4.2%
Cys[C] 0 0.0%
Gln[Q] 3 2.1%
Glu[E] 5 3.5%
Gly[G] 5 3.5%
His[H] 1 0.7%
Ile[I] 7 4.9%
Leu[L] 11 7.7%
Lys[K] 22 15.5%
Met[M] 3 2.1%
Phe[F] 2 1.4%
Pro[P] 6 4.2%
Ser[S] 6 4.2%
Thr[T] 10 7.0%
Trp[W] 0 0.0%
Tyr[Y] 8 5.6%
Val[V] 14 9.9%
Total Length 142

Instability index
Instability index (II) 26.36
This classifies the protein as stable

Atomic composition
Carbon 710
Hydrogen 1181
Nitrogen 197
Oxygen 199
Sulfur 3
Total number of atoms 2290
Formula = C710H1181N197O199S3

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 11920 M-1 cm-1

Aliphatic index
Aliphatic index 91.41

Coding Region Translation Calculations
Codon Bias 0.807
Codon Adaptation Index 0.748
Frequency of Optimal Codons 0.885
Hydropathicity of Protein -0.354
Aromaticity Score 0.070