HRP1/YOL123W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 24 4.5%
Arg[R] 28 5.2%
Asn[N] 54 10.1%
Asp[D] 45 8.4%
Cys[C] 2 0.4%
Gln[Q] 48 9.0%
Glu[E] 26 4.9%
Gly[G] 51 9.6%
His[H] 6 1.1%
Ile[I] 14 2.6%
Leu[L] 19 3.6%
Lys[K] 34 6.4%
Met[M] 24 4.5%
Phe[F] 18 3.4%
Pro[P] 29 5.4%
Ser[S] 48 9.0%
Thr[T] 29 5.4%
Trp[W] 3 0.6%
Tyr[Y] 19 3.6%
Val[V] 13 2.4%
Total Length 534

Instability index
Instability index (II) 49.26
This classifies the protein as unstable

Atomic composition
Carbon 2508
Hydrogen 3891
Nitrogen 769
Oxygen 875
Sulfur 26
Total number of atoms 8069
Formula = C2508H3891N769O875S26

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 44935 M-1 cm-1
assuming NO Cys residues appear as half cystines 44810 M-1 cm-1
assuming all Cys residues are reduced 44810 M-1 cm-1
assuming all pairs of Cys residues form cystines 44935 M-1 cm-1

Aliphatic index
Aliphatic index 35.66

Coding Region Translation Calculations
Codon Bias 0.225
Codon Adaptation Index 0.243
Frequency of Optimal Codons 0.540
Hydropathicity of Protein -1.315
Aromaticity Score 0.075