RPS19A/YOL121C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 13 9.0%
Arg[R] 12 8.3%
Asn[N] 5 3.5%
Asp[D] 8 5.6%
Cys[C] 0 0.0%
Gln[Q] 8 5.6%
Glu[E] 9 6.2%
Gly[G] 15 10.4%
His[H] 2 1.4%
Ile[I] 10 6.9%
Leu[L] 8 5.6%
Lys[K] 11 7.6%
Met[M] 3 2.1%
Phe[F] 3 2.1%
Pro[P] 6 4.2%
Ser[S] 10 6.9%
Thr[T] 2 1.4%
Trp[W] 1 0.7%
Tyr[Y] 6 4.2%
Val[V] 12 8.3%
Total Length 144

Instability index
Instability index (II) 53.43
This classifies the protein as unstable

Atomic composition
Carbon 699
Hydrogen 1129
Nitrogen 209
Oxygen 210
Sulfur 3
Total number of atoms 2250
Formula = C699H1129N209O210S3

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 14440 M-1 cm-1

Aliphatic index
Aliphatic index 81.94

Coding Region Translation Calculations
Codon Bias 0.789
Codon Adaptation Index 0.794
Frequency of Optimal Codons 0.871
Hydropathicity of Protein -0.547
Aromaticity Score 0.069