Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
32 |
5.5% |
| Arg[R] |
25 |
4.3% |
| Asn[N] |
33 |
5.7% |
| Asp[D] |
49 |
8.4% |
| Cys[C] |
3 |
0.5% |
| Gln[Q] |
12 |
2.1% |
| Glu[E] |
54 |
9.2% |
| Gly[G] |
31 |
5.3% |
| His[H] |
18 |
3.1% |
| Ile[I] |
46 |
7.9% |
| Leu[L] |
45 |
7.7% |
| Lys[K] |
54 |
9.2% |
| Met[M] |
2 |
0.3% |
| Phe[F] |
24 |
4.1% |
| Pro[P] |
25 |
4.3% |
| Ser[S] |
48 |
8.2% |
| Thr[T] |
32 |
5.5% |
| Trp[W] |
5 |
0.9% |
| Tyr[Y] |
16 |
2.7% |
| Val[V] |
30 |
5.1% |
| Total Length |
584 |
|
| Instability index (II) |
41.59 |
| This classifies the protein as unstable |
|
| Carbon |
2925 |
| Hydrogen |
4599 |
| Nitrogen |
799 |
| Oxygen |
932 |
| Sulfur |
5 |
| Total number of atoms |
9260 |
| Formula = C2925H4599N799O932S5 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
51465 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
51340 M-1 cm-1 |
| assuming all Cys residues are reduced |
51340 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
51465 M-1 cm-1 |
|
|
| Codon Bias |
-0.002 |
| Codon Adaptation Index |
0.148 |
| Frequency of Optimal Codons |
0.419 |
| Hydropathicity of Protein |
-0.680 |
| Aromaticity Score |
0.077 |
|
