MDY2/YOL111C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 15 7.1%
Arg[R] 3 1.4%
Asn[N] 10 4.7%
Asp[D] 10 4.7%
Cys[C] 0 0.0%
Gln[Q] 12 5.7%
Glu[E] 14 6.6%
Gly[G] 5 2.4%
His[H] 7 3.3%
Ile[I] 11 5.2%
Leu[L] 26 12.3%
Lys[K] 26 12.3%
Met[M] 3 1.4%
Phe[F] 6 2.8%
Pro[P] 17 8.0%
Ser[S] 17 8.0%
Thr[T] 15 7.1%
Trp[W] 2 0.9%
Tyr[Y] 3 1.4%
Val[V] 10 4.7%
Total Length 212

Instability index
Instability index (II) 39.72
This classifies the protein as stable

Atomic composition
Carbon 1067
Hydrogen 1727
Nitrogen 285
Oxygen 318
Sulfur 3
Total number of atoms 3400
Formula = C1067H1727N285O318S3

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 15470 M-1 cm-1

Aliphatic index
Aliphatic index 88.82

Coding Region Translation Calculations
Codon Bias 0.125
Codon Adaptation Index 0.171
Frequency of Optimal Codons 0.478
Hydropathicity of Protein -0.554
Aromaticity Score 0.052