INO4/YOL108C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 5 3.3%
Arg[R] 11 7.3%
Asn[N] 7 4.6%
Asp[D] 7 4.6%
Cys[C] 0 0.0%
Gln[Q] 10 6.6%
Glu[E] 16 10.6%
Gly[G] 8 5.3%
His[H] 2 1.3%
Ile[I] 15 9.9%
Leu[L] 15 9.9%
Lys[K] 17 11.3%
Met[M] 1 0.7%
Phe[F] 1 0.7%
Pro[P] 5 3.3%
Ser[S] 15 9.9%
Thr[T] 4 2.6%
Trp[W] 2 1.3%
Tyr[Y] 3 2.0%
Val[V] 7 4.6%
Total Length 151

Instability index
Instability index (II) 63.23
This classifies the protein as unstable

Atomic composition
Carbon 761
Hydrogen 1266
Nitrogen 224
Oxygen 237
Sulfur 1
Total number of atoms 2489
Formula = C761H1266N224O237S1

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 15470 M-1 cm-1

Aliphatic index
Aliphatic index 94.24

Coding Region Translation Calculations
Codon Bias -0.021
Codon Adaptation Index 0.083
Frequency of Optimal Codons 0.399
Hydropathicity of Protein -0.836
Aromaticity Score 0.040