Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
55 |
5.1% |
| Arg[R] |
47 |
4.3% |
| Asn[N] |
73 |
6.8% |
| Asp[D] |
61 |
5.6% |
| Cys[C] |
5 |
0.5% |
| Gln[Q] |
40 |
3.7% |
| Glu[E] |
51 |
4.7% |
| Gly[G] |
43 |
4.0% |
| His[H] |
19 |
1.8% |
| Ile[I] |
62 |
5.7% |
| Leu[L] |
88 |
8.1% |
| Lys[K] |
120 |
11.1% |
| Met[M] |
24 |
2.2% |
| Phe[F] |
38 |
3.5% |
| Pro[P] |
63 |
5.8% |
| Ser[S] |
121 |
11.2% |
| Thr[T] |
85 |
7.9% |
| Trp[W] |
3 |
0.3% |
| Tyr[Y] |
41 |
3.8% |
| Val[V] |
42 |
3.9% |
| Total Length |
1081 |
|
| Instability index (II) |
44.53 |
| This classifies the protein as unstable |
|
| Carbon |
5365 |
| Hydrogen |
8614 |
| Nitrogen |
1496 |
| Oxygen |
1666 |
| Sulfur |
29 |
| Total number of atoms |
17170 |
| Formula = C5365H8614N1496O1666S29 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
77840 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
77590 M-1 cm-1 |
| assuming all Cys residues are reduced |
77590 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
77840 M-1 cm-1 |
|
|
| Codon Bias |
0.016 |
| Codon Adaptation Index |
0.135 |
| Frequency of Optimal Codons |
0.419 |
| Hydropathicity of Protein |
-0.744 |
| Aromaticity Score |
0.076 |
|
