Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
58 |
5.9% |
| Arg[R] |
49 |
4.9% |
| Asn[N] |
50 |
5.0% |
| Asp[D] |
47 |
4.7% |
| Cys[C] |
13 |
1.3% |
| Gln[Q] |
28 |
2.8% |
| Glu[E] |
53 |
5.3% |
| Gly[G] |
52 |
5.2% |
| His[H] |
15 |
1.5% |
| Ile[I] |
61 |
6.2% |
| Leu[L] |
107 |
10.8% |
| Lys[K] |
63 |
6.4% |
| Met[M] |
20 |
2.0% |
| Phe[F] |
54 |
5.4% |
| Pro[P] |
56 |
5.7% |
| Ser[S] |
75 |
7.6% |
| Thr[T] |
56 |
5.7% |
| Trp[W] |
13 |
1.3% |
| Tyr[Y] |
49 |
4.9% |
| Val[V] |
72 |
7.3% |
| Total Length |
991 |
|
| Instability index (II) |
39.07 |
| This classifies the protein as stable |
|
| Carbon |
5139 |
| Hydrogen |
7984 |
| Nitrogen |
1322 |
| Oxygen |
1450 |
| Sulfur |
33 |
| Total number of atoms |
15928 |
| Formula = C5139H7984N1322O1450S33 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
145260 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
144510 M-1 cm-1 |
| assuming all Cys residues are reduced |
144510 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
145260 M-1 cm-1 |
|
|
| Codon Bias |
0.170 |
| Codon Adaptation Index |
0.157 |
| Frequency of Optimal Codons |
0.502 |
| Hydropathicity of Protein |
-0.114 |
| Aromaticity Score |
0.117 |
|
