Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
147 |
4.8% |
| Arg[R] |
136 |
4.4% |
| Asn[N] |
189 |
6.1% |
| Asp[D] |
157 |
5.1% |
| Cys[C] |
38 |
1.2% |
| Gln[Q] |
97 |
3.2% |
| Glu[E] |
179 |
5.8% |
| Gly[G] |
93 |
3.0% |
| His[H] |
101 |
3.3% |
| Ile[I] |
258 |
8.4% |
| Leu[L] |
339 |
11.0% |
| Lys[K] |
179 |
5.8% |
| Met[M] |
79 |
2.6% |
| Phe[F] |
171 |
5.6% |
| Pro[P] |
117 |
3.8% |
| Ser[S] |
332 |
10.8% |
| Thr[T] |
197 |
6.4% |
| Trp[W] |
20 |
0.6% |
| Tyr[Y] |
109 |
3.5% |
| Val[V] |
141 |
4.6% |
| Total Length |
3079 |
|
| Instability index (II) |
46.04 |
| This classifies the protein as unstable |
|
| Carbon |
15792 |
| Hydrogen |
24766 |
| Nitrogen |
4174 |
| Oxygen |
4676 |
| Sulfur |
117 |
| Total number of atoms |
49525 |
| Formula = C15792H24766N4174O4676S117 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
274785 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
272410 M-1 cm-1 |
| assuming all Cys residues are reduced |
272410 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
274785 M-1 cm-1 |
|
|
| Codon Bias |
0.055 |
| Codon Adaptation Index |
0.139 |
| Frequency of Optimal Codons |
0.445 |
| Hydropathicity of Protein |
-0.184 |
| Aromaticity Score |
0.097 |
|
