Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
29 |
3.6% |
| Arg[R] |
26 |
3.3% |
| Asn[N] |
58 |
7.3% |
| Asp[D] |
47 |
5.9% |
| Cys[C] |
14 |
1.8% |
| Gln[Q] |
28 |
3.5% |
| Glu[E] |
56 |
7.0% |
| Gly[G] |
24 |
3.0% |
| His[H] |
17 |
2.1% |
| Ile[I] |
52 |
6.5% |
| Leu[L] |
113 |
14.2% |
| Lys[K] |
79 |
9.9% |
| Met[M] |
16 |
2.0% |
| Phe[F] |
40 |
5.0% |
| Pro[P] |
19 |
2.4% |
| Ser[S] |
69 |
8.7% |
| Thr[T] |
33 |
4.1% |
| Trp[W] |
7 |
0.9% |
| Tyr[Y] |
40 |
5.0% |
| Val[V] |
29 |
3.6% |
| Total Length |
796 |
|
| Instability index (II) |
42.45 |
| This classifies the protein as unstable |
|
| Carbon |
4195 |
| Hydrogen |
6586 |
| Nitrogen |
1080 |
| Oxygen |
1231 |
| Sulfur |
30 |
| Total number of atoms |
13122 |
| Formula = C4195H6586N1080O1231S30 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
98975 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
98100 M-1 cm-1 |
| assuming all Cys residues are reduced |
98100 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
98975 M-1 cm-1 |
|
|
| Codon Bias |
0.070 |
| Codon Adaptation Index |
0.159 |
| Frequency of Optimal Codons |
0.454 |
| Hydropathicity of Protein |
-0.380 |
| Aromaticity Score |
0.109 |
|
