EMI5/YOL071W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 9 5.6%
Arg[R] 8 4.9%
Asn[N] 8 4.9%
Asp[D] 12 7.4%
Cys[C] 1 0.6%
Gln[Q] 6 3.7%
Glu[E] 12 7.4%
Gly[G] 5 3.1%
His[H] 2 1.2%
Ile[I] 9 5.6%
Leu[L] 20 12.3%
Lys[K] 19 11.7%
Met[M] 4 2.5%
Phe[F] 5 3.1%
Pro[P] 6 3.7%
Ser[S] 14 8.6%
Thr[T] 8 4.9%
Trp[W] 5 3.1%
Tyr[Y] 7 4.3%
Val[V] 2 1.2%
Total Length 162

Instability index
Instability index (II) 57.99
This classifies the protein as unstable

Atomic composition
Carbon 855
Hydrogen 1344
Nitrogen 228
Oxygen 254
Sulfur 5
Total number of atoms 2686
Formula = C855H1344N228O254S5

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 37930 M-1 cm-1
assuming NO Cys residues appear as half cystines 37930 M-1 cm-1
assuming all Cys residues are reduced 37930 M-1 cm-1
assuming all pairs of Cys residues form cystines 37930 M-1 cm-1

Aliphatic index
Aliphatic index 78.95

Coding Region Translation Calculations
Codon Bias 0.127
Codon Adaptation Index 0.149
Frequency of Optimal Codons 0.477
Hydropathicity of Protein -0.780
Aromaticity Score 0.105