GAL11/YOL051W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 107 9.9%
Arg[R] 34 3.1%
Asn[N] 118 10.9%
Asp[D] 34 3.1%
Cys[C] 4 0.4%
Gln[Q] 173 16.0%
Glu[E] 37 3.4%
Gly[G] 30 2.8%
His[H] 13 1.2%
Ile[I] 59 5.5%
Leu[L] 69 6.4%
Lys[K] 77 7.1%
Met[M] 33 3.1%
Phe[F] 25 2.3%
Pro[P] 55 5.1%
Ser[S] 88 8.1%
Thr[T] 61 5.6%
Trp[W] 5 0.5%
Tyr[Y] 13 1.2%
Val[V] 46 4.3%
Total Length 1081

Instability index
Instability index (II) 51.29
This classifies the protein as unstable

Atomic composition
Carbon 5138
Hydrogen 8354
Nitrogen 1582
Oxygen 1677
Sulfur 37
Total number of atoms 16788
Formula = C5138H8354N1582O1677S37

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 47120 M-1 cm-1
assuming NO Cys residues appear as half cystines 46870 M-1 cm-1
assuming all Cys residues are reduced 46870 M-1 cm-1
assuming all pairs of Cys residues form cystines 47120 M-1 cm-1

Aliphatic index
Aliphatic index 68.42

Coding Region Translation Calculations
Codon Bias 0.154
Codon Adaptation Index 0.161
Frequency of Optimal Codons 0.506
Hydropathicity of Protein -0.870
Aromaticity Score 0.040