Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
22 |
6.0% |
| Arg[R] |
16 |
4.4% |
| Asn[N] |
13 |
3.5% |
| Asp[D] |
15 |
4.1% |
| Cys[C] |
4 |
1.1% |
| Gln[Q] |
9 |
2.5% |
| Glu[E] |
24 |
6.5% |
| Gly[G] |
33 |
9.0% |
| His[H] |
5 |
1.4% |
| Ile[I] |
40 |
10.9% |
| Leu[L] |
29 |
7.9% |
| Lys[K] |
29 |
7.9% |
| Met[M] |
8 |
2.2% |
| Phe[F] |
13 |
3.5% |
| Pro[P] |
18 |
4.9% |
| Ser[S] |
31 |
8.4% |
| Thr[T] |
19 |
5.2% |
| Trp[W] |
4 |
1.1% |
| Tyr[Y] |
9 |
2.5% |
| Val[V] |
26 |
7.1% |
| Total Length |
367 |
|
| Instability index (II) |
40.33 |
| This classifies the protein as unstable |
|
| Carbon |
1806 |
| Hydrogen |
2916 |
| Nitrogen |
480 |
| Oxygen |
527 |
| Sulfur |
12 |
| Total number of atoms |
5741 |
| Formula = C1806H2916N480O527S12 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
35660 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
35410 M-1 cm-1 |
|
|
| Codon Bias |
0.154 |
| Codon Adaptation Index |
0.162 |
| Frequency of Optimal Codons |
0.501 |
| Hydropathicity of Protein |
-0.026 |
| Aromaticity Score |
0.071 |
|
