Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
86 |
5.6% |
| Arg[R] |
57 |
3.7% |
| Asn[N] |
90 |
5.9% |
| Asp[D] |
96 |
6.2% |
| Cys[C] |
9 |
0.6% |
| Gln[Q] |
116 |
7.6% |
| Glu[E] |
120 |
7.8% |
| Gly[G] |
59 |
3.8% |
| His[H] |
47 |
3.1% |
| Ile[I] |
95 |
6.2% |
| Leu[L] |
122 |
7.9% |
| Lys[K] |
105 |
6.8% |
| Met[M] |
24 |
1.6% |
| Phe[F] |
61 |
4.0% |
| Pro[P] |
98 |
6.4% |
| Ser[S] |
132 |
8.6% |
| Thr[T] |
87 |
5.7% |
| Trp[W] |
14 |
0.9% |
| Tyr[Y] |
49 |
3.2% |
| Val[V] |
69 |
4.5% |
| Total Length |
1536 |
|
| Instability index (II) |
53.40 |
| This classifies the protein as unstable |
|
| Carbon |
7726 |
| Hydrogen |
12024 |
| Nitrogen |
2126 |
| Oxygen |
2443 |
| Sulfur |
33 |
| Total number of atoms |
24352 |
| Formula = C7726H12024N2126O2443S33 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
150510 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
150010 M-1 cm-1 |
| assuming all Cys residues are reduced |
150010 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
150510 M-1 cm-1 |
|
|
| Codon Bias |
0.067 |
| Codon Adaptation Index |
0.150 |
| Frequency of Optimal Codons |
0.455 |
| Hydropathicity of Protein |
-0.743 |
| Aromaticity Score |
0.081 |
|
