Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
34 |
5.6% |
| Arg[R] |
20 |
3.3% |
| Asn[N] |
44 |
7.3% |
| Asp[D] |
27 |
4.5% |
| Cys[C] |
4 |
0.7% |
| Gln[Q] |
20 |
3.3% |
| Glu[E] |
24 |
4.0% |
| Gly[G] |
22 |
3.6% |
| His[H] |
12 |
2.0% |
| Ile[I] |
55 |
9.1% |
| Leu[L] |
62 |
10.2% |
| Lys[K] |
29 |
4.8% |
| Met[M] |
12 |
2.0% |
| Phe[F] |
35 |
5.8% |
| Pro[P] |
34 |
5.6% |
| Ser[S] |
75 |
12.4% |
| Thr[T] |
47 |
7.8% |
| Trp[W] |
6 |
1.0% |
| Tyr[Y] |
18 |
3.0% |
| Val[V] |
25 |
4.1% |
| Total Length |
605 |
|
| Instability index (II) |
43.80 |
| This classifies the protein as unstable |
|
| Carbon |
3041 |
| Hydrogen |
4756 |
| Nitrogen |
788 |
| Oxygen |
912 |
| Sulfur |
16 |
| Total number of atoms |
9513 |
| Formula = C3041H4756N788O912S16 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
60070 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
59820 M-1 cm-1 |
| assuming all Cys residues are reduced |
59820 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
60070 M-1 cm-1 |
|
|
| Codon Bias |
0.019 |
| Codon Adaptation Index |
0.116 |
| Frequency of Optimal Codons |
0.421 |
| Hydropathicity of Protein |
-0.081 |
| Aromaticity Score |
0.098 |
|
