Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
33 |
5.8% |
| Arg[R] |
21 |
3.7% |
| Asn[N] |
53 |
9.4% |
| Asp[D] |
35 |
6.2% |
| Cys[C] |
2 |
0.4% |
| Gln[Q] |
20 |
3.5% |
| Glu[E] |
44 |
7.8% |
| Gly[G] |
16 |
2.8% |
| His[H] |
13 |
2.3% |
| Ile[I] |
31 |
5.5% |
| Leu[L] |
55 |
9.7% |
| Lys[K] |
43 |
7.6% |
| Met[M] |
17 |
3.0% |
| Phe[F] |
21 |
3.7% |
| Pro[P] |
30 |
5.3% |
| Ser[S] |
56 |
9.9% |
| Thr[T] |
33 |
5.8% |
| Trp[W] |
4 |
0.7% |
| Tyr[Y] |
11 |
1.9% |
| Val[V] |
28 |
4.9% |
| Total Length |
566 |
|
| Instability index (II) |
41.20 |
| This classifies the protein as unstable |
|
| Carbon |
2794 |
| Hydrogen |
4445 |
| Nitrogen |
775 |
| Oxygen |
898 |
| Sulfur |
19 |
| Total number of atoms |
8931 |
| Formula = C2794H4445N775O898S19 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
38515 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
38390 M-1 cm-1 |
| assuming all Cys residues are reduced |
38390 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
38515 M-1 cm-1 |
|
|
| Codon Bias |
-0.001 |
| Codon Adaptation Index |
0.124 |
| Frequency of Optimal Codons |
0.409 |
| Hydropathicity of Protein |
-0.626 |
| Aromaticity Score |
0.064 |
|
