YNL338W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 0 0.0%
Arg[R] 1 1.9%
Asn[N] 2 3.8%
Asp[D] 1 1.9%
Cys[C] 1 1.9%
Gln[Q] 1 1.9%
Glu[E] 0 0.0%
Gly[G] 0 0.0%
His[H] 10 19.2%
Ile[I] 2 3.8%
Leu[L] 3 5.8%
Lys[K] 0 0.0%
Met[M] 2 3.8%
Phe[F] 1 1.9%
Pro[P] 11 21.2%
Ser[S] 4 7.7%
Thr[T] 11 21.2%
Trp[W] 0 0.0%
Tyr[Y] 2 3.8%
Val[V] 0 0.0%
Total Length 52

Instability index
Instability index (II) 26.96
This classifies the protein as stable

Atomic composition
Carbon 264
Hydrogen 388
Nitrogen 78
Oxygen 75
Sulfur 3
Total number of atoms 808
Formula = C264H388N78O75S3

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 2980 M-1 cm-1
assuming NO Cys residues appear as half cystines 2980 M-1 cm-1
assuming all Cys residues are reduced 2980 M-1 cm-1
assuming all pairs of Cys residues form cystines 2980 M-1 cm-1

Aliphatic index
Aliphatic index 37.50

Coding Region Translation Calculations
Codon Bias 0.218
Codon Adaptation Index 0.152
Frequency of Optimal Codons 0.540
Hydropathicity of Protein -1.002
Aromaticity Score 0.058