Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
79 |
8.7% |
| Arg[R] |
36 |
4.0% |
| Asn[N] |
33 |
3.7% |
| Asp[D] |
42 |
4.6% |
| Cys[C] |
18 |
2.0% |
| Gln[Q] |
39 |
4.3% |
| Glu[E] |
75 |
8.3% |
| Gly[G] |
37 |
4.1% |
| His[H] |
11 |
1.2% |
| Ile[I] |
57 |
6.3% |
| Leu[L] |
116 |
12.8% |
| Lys[K] |
65 |
7.2% |
| Met[M] |
15 |
1.7% |
| Phe[F] |
27 |
3.0% |
| Pro[P] |
38 |
4.2% |
| Ser[S] |
74 |
8.2% |
| Thr[T] |
45 |
5.0% |
| Trp[W] |
20 |
2.2% |
| Tyr[Y] |
38 |
4.2% |
| Val[V] |
39 |
4.3% |
| Total Length |
904 |
|
| Instability index (II) |
43.10 |
| This classifies the protein as unstable |
|
| Carbon |
4612 |
| Hydrogen |
7257 |
| Nitrogen |
1191 |
| Oxygen |
1368 |
| Sulfur |
33 |
| Total number of atoms |
14461 |
| Formula = C4612H7257N1191O1368S33 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
167745 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
166620 M-1 cm-1 |
| assuming all Cys residues are reduced |
166620 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
167745 M-1 cm-1 |
|
|
| Codon Bias |
0.128 |
| Codon Adaptation Index |
0.158 |
| Frequency of Optimal Codons |
0.472 |
| Hydropathicity of Protein |
-0.214 |
| Aromaticity Score |
0.094 |
|
