ZIM17/YNL310C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 12 6.9%
Arg[R] 9 5.2%
Asn[N] 8 4.6%
Asp[D] 10 5.7%
Cys[C] 6 3.4%
Gln[Q] 7 4.0%
Glu[E] 7 4.0%
Gly[G] 5 2.9%
His[H] 11 6.3%
Ile[I] 10 5.7%
Leu[L] 13 7.5%
Lys[K] 19 10.9%
Met[M] 5 2.9%
Phe[F] 5 2.9%
Pro[P] 9 5.2%
Ser[S] 11 6.3%
Thr[T] 10 5.7%
Trp[W] 1 0.6%
Tyr[Y] 4 2.3%
Val[V] 12 6.9%
Total Length 174

Instability index
Instability index (II) 31.55
This classifies the protein as stable

Atomic composition
Carbon 873
Hydrogen 1408
Nitrogen 258
Oxygen 249
Sulfur 11
Total number of atoms 2799
Formula = C873H1408N258O249S11

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 11835 M-1 cm-1
assuming NO Cys residues appear as half cystines 11460 M-1 cm-1
assuming all Cys residues are reduced 11460 M-1 cm-1
assuming all pairs of Cys residues form cystines 11835 M-1 cm-1

Aliphatic index
Aliphatic index 78.45

Coding Region Translation Calculations
Codon Bias 0.092
Codon Adaptation Index 0.137
Frequency of Optimal Codons 0.470
Hydropathicity of Protein -0.547
Aromaticity Score 0.057