Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
18 |
6.1% |
| Arg[R] |
9 |
3.0% |
| Asn[N] |
11 |
3.7% |
| Asp[D] |
10 |
3.4% |
| Cys[C] |
7 |
2.4% |
| Gln[Q] |
10 |
3.4% |
| Glu[E] |
15 |
5.1% |
| Gly[G] |
11 |
3.7% |
| His[H] |
4 |
1.3% |
| Ile[I] |
20 |
6.7% |
| Leu[L] |
44 |
14.8% |
| Lys[K] |
7 |
2.4% |
| Met[M] |
8 |
2.7% |
| Phe[F] |
14 |
4.7% |
| Pro[P] |
14 |
4.7% |
| Ser[S] |
35 |
11.8% |
| Thr[T] |
15 |
5.1% |
| Trp[W] |
8 |
2.7% |
| Tyr[Y] |
17 |
5.7% |
| Val[V] |
20 |
6.7% |
| Total Length |
297 |
|
| Instability index (II) |
50.26 |
| This classifies the protein as unstable |
|
| Carbon |
1552 |
| Hydrogen |
2374 |
| Nitrogen |
368 |
| Oxygen |
436 |
| Sulfur |
15 |
| Total number of atoms |
4745 |
| Formula = C1552H2374N368O436S15 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
69705 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
69330 M-1 cm-1 |
| assuming all Cys residues are reduced |
69330 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
69705 M-1 cm-1 |
|
|
| Codon Bias |
0.072 |
| Codon Adaptation Index |
0.100 |
| Frequency of Optimal Codons |
0.434 |
| Hydropathicity of Protein |
0.368 |
| Aromaticity Score |
0.131 |
|
