Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
36 |
7.2% |
| Arg[R] |
13 |
2.6% |
| Asn[N] |
22 |
4.4% |
| Asp[D] |
22 |
4.4% |
| Cys[C] |
9 |
1.8% |
| Gln[Q] |
19 |
3.8% |
| Glu[E] |
10 |
2.0% |
| Gly[G] |
25 |
5.0% |
| His[H] |
9 |
1.8% |
| Ile[I] |
17 |
3.4% |
| Leu[L] |
25 |
5.0% |
| Lys[K] |
17 |
3.4% |
| Met[M] |
4 |
0.8% |
| Phe[F] |
10 |
2.0% |
| Pro[P] |
16 |
3.2% |
| Ser[S] |
123 |
24.5% |
| Thr[T] |
73 |
14.5% |
| Trp[W] |
4 |
0.8% |
| Tyr[Y] |
18 |
3.6% |
| Val[V] |
31 |
6.2% |
| Total Length |
503 |
|
| Instability index (II) |
55.39 |
| This classifies the protein as unstable |
|
| Carbon |
2204 |
| Hydrogen |
3496 |
| Nitrogen |
622 |
| Oxygen |
823 |
| Sulfur |
13 |
| Total number of atoms |
7158 |
| Formula = C2204H3496N622O823S13 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
49320 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
48820 M-1 cm-1 |
| assuming all Cys residues are reduced |
48820 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
49320 M-1 cm-1 |
|
|
| Codon Bias |
0.175 |
| Codon Adaptation Index |
0.145 |
| Frequency of Optimal Codons |
0.505 |
| Hydropathicity of Protein |
-0.391 |
| Aromaticity Score |
0.064 |
|
