Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
64 |
5.2% |
| Arg[R] |
59 |
4.8% |
| Asn[N] |
72 |
5.8% |
| Asp[D] |
93 |
7.5% |
| Cys[C] |
7 |
0.6% |
| Gln[Q] |
47 |
3.8% |
| Glu[E] |
82 |
6.6% |
| Gly[G] |
49 |
4.0% |
| His[H] |
19 |
1.5% |
| Ile[I] |
73 |
5.9% |
| Leu[L] |
140 |
11.3% |
| Lys[K] |
99 |
8.0% |
| Met[M] |
23 |
1.9% |
| Phe[F] |
68 |
5.5% |
| Pro[P] |
44 |
3.6% |
| Ser[S] |
119 |
9.6% |
| Thr[T] |
64 |
5.2% |
| Trp[W] |
11 |
0.9% |
| Tyr[Y] |
36 |
2.9% |
| Val[V] |
69 |
5.6% |
| Total Length |
1238 |
|
| Instability index (II) |
43.01 |
| This classifies the protein as unstable |
|
| Carbon |
6306 |
| Hydrogen |
9948 |
| Nitrogen |
1682 |
| Oxygen |
1927 |
| Sulfur |
30 |
| Total number of atoms |
19893 |
| Formula = C6306H9948N1682O1927S30 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
114515 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
114140 M-1 cm-1 |
| assuming all Cys residues are reduced |
114140 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
114515 M-1 cm-1 |
|
|
| Codon Bias |
0.062 |
| Codon Adaptation Index |
0.147 |
| Frequency of Optimal Codons |
0.443 |
| Hydropathicity of Protein |
-0.413 |
| Aromaticity Score |
0.093 |
|
