Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
47 |
6.2% |
| Arg[R] |
29 |
3.8% |
| Asn[N] |
46 |
6.1% |
| Asp[D] |
65 |
8.6% |
| Cys[C] |
9 |
1.2% |
| Gln[Q] |
34 |
4.5% |
| Glu[E] |
65 |
8.6% |
| Gly[G] |
26 |
3.4% |
| His[H] |
11 |
1.4% |
| Ile[I] |
46 |
6.1% |
| Leu[L] |
67 |
8.8% |
| Lys[K] |
71 |
9.4% |
| Met[M] |
9 |
1.2% |
| Phe[F] |
17 |
2.2% |
| Pro[P] |
19 |
2.5% |
| Ser[S] |
101 |
13.3% |
| Thr[T] |
49 |
6.5% |
| Trp[W] |
7 |
0.9% |
| Tyr[Y] |
13 |
1.7% |
| Val[V] |
28 |
3.7% |
| Total Length |
759 |
|
| Instability index (II) |
56.00 |
| This classifies the protein as unstable |
|
| Carbon |
3629 |
| Hydrogen |
5862 |
| Nitrogen |
1026 |
| Oxygen |
1263 |
| Sulfur |
18 |
| Total number of atoms |
11798 |
| Formula = C3629H5862N1026O1263S18 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
58370 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
57870 M-1 cm-1 |
| assuming all Cys residues are reduced |
57870 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
58370 M-1 cm-1 |
|
|
| Codon Bias |
0.050 |
| Codon Adaptation Index |
0.148 |
| Frequency of Optimal Codons |
0.443 |
| Hydropathicity of Protein |
-0.798 |
| Aromaticity Score |
0.049 |
|
