ATX1/YNL259C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 2 2.7%
Arg[R] 1 1.4%
Asn[N] 2 2.7%
Asp[D] 4 5.5%
Cys[C] 2 2.7%
Gln[Q] 3 4.1%
Glu[E] 5 6.8%
Gly[G] 4 5.5%
His[H] 1 1.4%
Ile[I] 5 6.8%
Leu[L] 7 9.6%
Lys[K] 10 13.7%
Met[M] 2 2.7%
Phe[F] 2 2.7%
Pro[P] 2 2.7%
Ser[S] 5 6.8%
Thr[T] 5 6.8%
Trp[W] 0 0.0%
Tyr[Y] 3 4.1%
Val[V] 8 11.0%
Total Length 73

Instability index
Instability index (II) 27.74
This classifies the protein as stable

Atomic composition
Carbon 368
Hydrogen 605
Nitrogen 93
Oxygen 110
Sulfur 4
Total number of atoms 1180
Formula = C368H605N93O110S4

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 4595 M-1 cm-1
assuming NO Cys residues appear as half cystines 4470 M-1 cm-1
assuming all Cys residues are reduced 4470 M-1 cm-1
assuming all pairs of Cys residues form cystines 4595 M-1 cm-1

Aliphatic index
Aliphatic index 98.63

Coding Region Translation Calculations
Codon Bias 0.104
Codon Adaptation Index 0.169
Frequency of Optimal Codons 0.493
Hydropathicity of Protein -0.153
Aromaticity Score 0.068