Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
49 |
3.7% |
| Arg[R] |
59 |
4.5% |
| Asn[N] |
92 |
7.0% |
| Asp[D] |
97 |
7.4% |
| Cys[C] |
9 |
0.7% |
| Gln[Q] |
79 |
6.0% |
| Glu[E] |
132 |
10.1% |
| Gly[G] |
29 |
2.2% |
| His[H] |
22 |
1.7% |
| Ile[I] |
101 |
7.7% |
| Leu[L] |
153 |
11.7% |
| Lys[K] |
149 |
11.4% |
| Met[M] |
27 |
2.1% |
| Phe[F] |
39 |
3.0% |
| Pro[P] |
14 |
1.1% |
| Ser[S] |
108 |
8.2% |
| Thr[T] |
73 |
5.6% |
| Trp[W] |
5 |
0.4% |
| Tyr[Y] |
28 |
2.1% |
| Val[V] |
47 |
3.6% |
| Total Length |
1312 |
|
| Instability index (II) |
43.28 |
| This classifies the protein as unstable |
|
| Carbon |
6661 |
| Hydrogen |
10884 |
| Nitrogen |
1858 |
| Oxygen |
2151 |
| Sulfur |
36 |
| Total number of atoms |
21590 |
| Formula = C6661H10884N1858O2151S36 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
69720 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
69220 M-1 cm-1 |
| assuming all Cys residues are reduced |
69220 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
69720 M-1 cm-1 |
|
|
| Codon Bias |
0.084 |
| Codon Adaptation Index |
0.171 |
| Frequency of Optimal Codons |
0.474 |
| Hydropathicity of Protein |
-0.781 |
| Aromaticity Score |
0.055 |
|
