Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
50 |
6.1% |
| Arg[R] |
32 |
3.9% |
| Asn[N] |
45 |
5.5% |
| Asp[D] |
66 |
8.1% |
| Cys[C] |
8 |
1.0% |
| Gln[Q] |
16 |
2.0% |
| Glu[E] |
53 |
6.5% |
| Gly[G] |
57 |
7.0% |
| His[H] |
22 |
2.7% |
| Ile[I] |
48 |
5.9% |
| Leu[L] |
62 |
7.6% |
| Lys[K] |
41 |
5.0% |
| Met[M] |
10 |
1.2% |
| Phe[F] |
32 |
3.9% |
| Pro[P] |
36 |
4.4% |
| Ser[S] |
92 |
11.3% |
| Thr[T] |
55 |
6.8% |
| Trp[W] |
15 |
1.8% |
| Tyr[Y] |
28 |
3.4% |
| Val[V] |
46 |
5.7% |
| Total Length |
814 |
|
| Instability index (II) |
38.07 |
| This classifies the protein as stable |
|
| Carbon |
3968 |
| Hydrogen |
6093 |
| Nitrogen |
1071 |
| Oxygen |
1289 |
| Sulfur |
18 |
| Total number of atoms |
12439 |
| Formula = C3968H6093N1071O1289S18 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
124720 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
124220 M-1 cm-1 |
| assuming all Cys residues are reduced |
124220 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
124720 M-1 cm-1 |
|
|
| Codon Bias |
0.074 |
| Codon Adaptation Index |
0.150 |
| Frequency of Optimal Codons |
0.450 |
| Hydropathicity of Protein |
-0.471 |
| Aromaticity Score |
0.092 |
|
