Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
45 |
4.6% |
| Arg[R] |
33 |
3.4% |
| Asn[N] |
63 |
6.5% |
| Asp[D] |
46 |
4.7% |
| Cys[C] |
14 |
1.4% |
| Gln[Q] |
46 |
4.7% |
| Glu[E] |
49 |
5.0% |
| Gly[G] |
32 |
3.3% |
| His[H] |
20 |
2.1% |
| Ile[I] |
75 |
7.7% |
| Leu[L] |
98 |
10.1% |
| Lys[K] |
74 |
7.6% |
| Met[M] |
21 |
2.2% |
| Phe[F] |
54 |
5.5% |
| Pro[P] |
46 |
4.7% |
| Ser[S] |
96 |
9.9% |
| Thr[T] |
62 |
6.4% |
| Trp[W] |
13 |
1.3% |
| Tyr[Y] |
36 |
3.7% |
| Val[V] |
51 |
5.2% |
| Total Length |
974 |
|
| Instability index (II) |
46.19 |
| This classifies the protein as unstable |
|
| Carbon |
5031 |
| Hydrogen |
7863 |
| Nitrogen |
1309 |
| Oxygen |
1468 |
| Sulfur |
35 |
| Total number of atoms |
15706 |
| Formula = C5031H7863N1309O1468S35 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
126015 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
125140 M-1 cm-1 |
| assuming all Cys residues are reduced |
125140 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
126015 M-1 cm-1 |
|
|
| Codon Bias |
0.005 |
| Codon Adaptation Index |
0.126 |
| Frequency of Optimal Codons |
0.420 |
| Hydropathicity of Protein |
-0.256 |
| Aromaticity Score |
0.106 |
|
