Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
35 |
3.9% |
| Arg[R] |
45 |
5.0% |
| Asn[N] |
58 |
6.5% |
| Asp[D] |
75 |
8.4% |
| Cys[C] |
3 |
0.3% |
| Gln[Q] |
32 |
3.6% |
| Glu[E] |
82 |
9.2% |
| Gly[G] |
26 |
2.9% |
| His[H] |
16 |
1.8% |
| Ile[I] |
46 |
5.2% |
| Leu[L] |
67 |
7.5% |
| Lys[K] |
68 |
7.6% |
| Met[M] |
17 |
1.9% |
| Phe[F] |
35 |
3.9% |
| Pro[P] |
49 |
5.5% |
| Ser[S] |
120 |
13.5% |
| Thr[T] |
63 |
7.1% |
| Trp[W] |
0 |
0.0% |
| Tyr[Y] |
19 |
2.1% |
| Val[V] |
36 |
4.0% |
| Total Length |
892 |
|
| Instability index (II) |
54.88 |
| This classifies the protein as unstable |
|
| Carbon |
4328 |
| Hydrogen |
6881 |
| Nitrogen |
1217 |
| Oxygen |
1499 |
| Sulfur |
20 |
| Total number of atoms |
13945 |
| Formula = C4328H6881N1217O1499S20 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
28435 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
28310 M-1 cm-1 |
| assuming all Cys residues are reduced |
28310 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
28435 M-1 cm-1 |
|
|
| Codon Bias |
0.046 |
| Codon Adaptation Index |
0.138 |
| Frequency of Optimal Codons |
0.439 |
| Hydropathicity of Protein |
-0.923 |
| Aromaticity Score |
0.061 |
|
