URE2/YNL229C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 25 7.1%
Arg[R] 19 5.4%
Asn[N] 46 13.0%
Asp[D] 15 4.2%
Cys[C] 0 0.0%
Gln[Q] 18 5.1%
Glu[E] 21 5.9%
Gly[G] 19 5.4%
His[H] 11 3.1%
Ile[I] 18 5.1%
Leu[L] 26 7.3%
Lys[K] 11 3.1%
Met[M] 10 2.8%
Phe[F] 19 5.4%
Pro[P] 12 3.4%
Ser[S] 27 7.6%
Thr[T] 16 4.5%
Trp[W] 6 1.7%
Tyr[Y] 11 3.1%
Val[V] 24 6.8%
Total Length 354

Instability index
Instability index (II) 38.83
This classifies the protein as stable

Atomic composition
Carbon 1773
Hydrogen 2714
Nitrogen 514
Oxygen 545
Sulfur 10
Total number of atoms 5556
Formula = C1773H2714N514O545S10

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 49390 M-1 cm-1

Aliphatic index
Aliphatic index 75.20

Coding Region Translation Calculations
Codon Bias 0.023
Codon Adaptation Index 0.125
Frequency of Optimal Codons 0.423
Hydropathicity of Protein -0.551
Aromaticity Score 0.102