Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
48 |
5.5% |
| Arg[R] |
65 |
7.4% |
| Asn[N] |
52 |
5.9% |
| Asp[D] |
44 |
5.0% |
| Cys[C] |
17 |
1.9% |
| Gln[Q] |
39 |
4.5% |
| Glu[E] |
39 |
4.5% |
| Gly[G] |
42 |
4.8% |
| His[H] |
23 |
2.6% |
| Ile[I] |
56 |
6.4% |
| Leu[L] |
82 |
9.4% |
| Lys[K] |
76 |
8.7% |
| Met[M] |
20 |
2.3% |
| Phe[F] |
33 |
3.8% |
| Pro[P] |
38 |
4.3% |
| Ser[S] |
77 |
8.8% |
| Thr[T] |
47 |
5.4% |
| Trp[W] |
13 |
1.5% |
| Tyr[Y] |
27 |
3.1% |
| Val[V] |
37 |
4.2% |
| Total Length |
875 |
|
| Instability index (II) |
42.83 |
| This classifies the protein as unstable |
|
| Carbon |
4442 |
| Hydrogen |
7104 |
| Nitrogen |
1296 |
| Oxygen |
1284 |
| Sulfur |
37 |
| Total number of atoms |
14163 |
| Formula = C4442H7104N1296O1284S37 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
112730 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
111730 M-1 cm-1 |
| assuming all Cys residues are reduced |
111730 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
112730 M-1 cm-1 |
|
|
| Codon Bias |
0.033 |
| Codon Adaptation Index |
0.124 |
| Frequency of Optimal Codons |
0.419 |
| Hydropathicity of Protein |
-0.585 |
| Aromaticity Score |
0.083 |
|
