Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
6 |
3.0% |
| Arg[R] |
14 |
7.0% |
| Asn[N] |
17 |
8.5% |
| Asp[D] |
9 |
4.5% |
| Cys[C] |
0 |
0.0% |
| Gln[Q] |
17 |
8.5% |
| Glu[E] |
10 |
5.0% |
| Gly[G] |
10 |
5.0% |
| His[H] |
1 |
0.5% |
| Ile[I] |
19 |
9.5% |
| Leu[L] |
14 |
7.0% |
| Lys[K] |
9 |
4.5% |
| Met[M] |
6 |
3.0% |
| Phe[F] |
13 |
6.5% |
| Pro[P] |
7 |
3.5% |
| Ser[S] |
17 |
8.5% |
| Thr[T] |
9 |
4.5% |
| Trp[W] |
3 |
1.5% |
| Tyr[Y] |
6 |
3.0% |
| Val[V] |
12 |
6.0% |
| Total Length |
199 |
|
| Instability index (II) |
54.70 |
| This classifies the protein as unstable |
|
| Carbon |
1035 |
| Hydrogen |
1621 |
| Nitrogen |
289 |
| Oxygen |
304 |
| Sulfur |
6 |
| Total number of atoms |
3255 |
| Formula = C1035H1621N289O304S6 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
25440 M-1 cm-1 |
|
|
| Codon Bias |
0.025 |
| Codon Adaptation Index |
0.117 |
| Frequency of Optimal Codons |
0.437 |
| Hydropathicity of Protein |
-0.426 |
| Aromaticity Score |
0.111 |
|
