Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
61 |
10.0% |
| Arg[R] |
29 |
4.7% |
| Asn[N] |
22 |
3.6% |
| Asp[D] |
38 |
6.2% |
| Cys[C] |
2 |
0.3% |
| Gln[Q] |
25 |
4.1% |
| Glu[E] |
49 |
8.0% |
| Gly[G] |
42 |
6.9% |
| His[H] |
5 |
0.8% |
| Ile[I] |
36 |
5.9% |
| Leu[L] |
51 |
8.3% |
| Lys[K] |
47 |
7.7% |
| Met[M] |
9 |
1.5% |
| Phe[F] |
26 |
4.2% |
| Pro[P] |
20 |
3.3% |
| Ser[S] |
44 |
7.2% |
| Thr[T] |
45 |
7.3% |
| Trp[W] |
1 |
0.2% |
| Tyr[Y] |
9 |
1.5% |
| Val[V] |
52 |
8.5% |
| Total Length |
613 |
|
| Instability index (II) |
36.15 |
| This classifies the protein as stable |
|
| Carbon |
2934 |
| Hydrogen |
4761 |
| Nitrogen |
805 |
| Oxygen |
933 |
| Sulfur |
11 |
| Total number of atoms |
9444 |
| Formula = C2934H4761N805O933S11 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
19035 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
18910 M-1 cm-1 |
| assuming all Cys residues are reduced |
18910 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
19035 M-1 cm-1 |
|
|
| Codon Bias |
0.808 |
| Codon Adaptation Index |
0.772 |
| Frequency of Optimal Codons |
0.886 |
| Hydropathicity of Protein |
-0.241 |
| Aromaticity Score |
0.059 |
|
